The P. putida Genome Oligonucleotide Array is a powerful tool for the analysis of gene expression in Pseudomonas putida (strain KT2440), a widely studied organism because of its potent catabolic potential that is capable of decontaminating organic substances including solvents such as toluene.
Strain KT2440 (a certified biosafety strain) is used worldwide as a workhorse for genetic and physiological studies and is being exploited in the development of a variety of biotechnological applications. Changes in gene expression could explain this extremely versatile metabolism.
The P. putida Genome Oligonucletotide Array provides you the opportunity to study the global expression and regulation of 5539 individual ORFs in a single experiment.
Progenika Biopharma develops oligonucleotide DNA-chips both for analyzing eukaryotic and prokaryotic gene expression. DNA-Chips are based in a glass-based oligonucleotide-array that can detects the transcripts of interest.
Each gene is represented by a 50-mer sense oligonucleotide that hybridizes close to the 3’ end of the target gene; oligonucleotide sequences are selected for their minimal homology with other genes in the array (less than 70% homology to any entry in GeneBank), in order to reduce the risk of non-specific hybridization.
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